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KMID : 1041219960380020144
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Journal of Sericultural and Entomological Science
1996 Volume.38 No. 2 p.144 ~ p.149
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Characterization of Spodoptera exigua Nuclear Polyhedrosis Virus Polyhedrin Gene Structure
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Abstract
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To develope the baculovirus expression vector system (BEVS) using Spodoptera exigua nuclear polyhedrosis virus (SeNPV), We characterized the polyhedrin of SeNPV. The SeNPV polyhedra was irregular and composed of the major protein of molecular weight of 30 kDa determined by electronmicroscopy and SDR-PAGE analysis, respectively. The nucleotide sequences of 876 bases including the coding region of polyhedirin gene was determined and it was revealed that the polyhedrin gene is located within Xho I 3.0 Kb and Nco I 6.0 Kb by southern blot analysis, respectively. Also, the Xho I 3.0 Kb and the Nco I 6.0Kb fragments were cloned and restriction enzyme map of these clones were determined.
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